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incucyte zoom integrated software  (Sartorius AG)
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Motility, proliferation, and invasiveness of SCC-25 cells after exposure to monocyte- and macrophages-conditioned medium. (A) Relative wound density curve of SCC-25 cells over 40 hours as measured by <t>IncuCyte</t> analysis. (B) Representative images of scratch assays showing wounds immediately after scratching (0h) and after 12 hours in the presence of monocyte- and macrophage-CM (right panels) versus control medium (left panels). Scale bars represent 300µm. (C) Proliferation curve for SCC-25 cells in the presence of monocyte and macrophages-derived conditioned medium (M1-CM and M2-CM). Data are presented as mean ± standard error of the mean (SEM) from a single experiment and are representative of at least two experiments. (D) Transwell invasion assay for SCC-25 cells at 48 hours post-incubation with monocyte- and macrophage-CM. Data are shown as mean ± SEM of cells counted in five representative microscopic fields per membrane using the ImageJ software. *P <0.05; **P<0.01
Incucyte Zoom Integrated Software, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Motility, proliferation, and invasiveness of SCC-25 cells after exposure to monocyte- and macrophages-conditioned medium. (A) Relative wound density curve of SCC-25 cells over 40 hours as measured by <t>IncuCyte</t> analysis. (B) Representative images of scratch assays showing wounds immediately after scratching (0h) and after 12 hours in the presence of monocyte- and macrophage-CM (right panels) versus control medium (left panels). Scale bars represent 300µm. (C) Proliferation curve for SCC-25 cells in the presence of monocyte and macrophages-derived conditioned medium (M1-CM and M2-CM). Data are presented as mean ± standard error of the mean (SEM) from a single experiment and are representative of at least two experiments. (D) Transwell invasion assay for SCC-25 cells at 48 hours post-incubation with monocyte- and macrophage-CM. Data are shown as mean ± SEM of cells counted in five representative microscopic fields per membrane using the ImageJ software. *P <0.05; **P<0.01
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Motility, proliferation, and invasiveness of SCC-25 cells after exposure to monocyte- and macrophages-conditioned medium. (A) Relative wound density curve of SCC-25 cells over 40 hours as measured by <t>IncuCyte</t> analysis. (B) Representative images of scratch assays showing wounds immediately after scratching (0h) and after 12 hours in the presence of monocyte- and macrophage-CM (right panels) versus control medium (left panels). Scale bars represent 300µm. (C) Proliferation curve for SCC-25 cells in the presence of monocyte and macrophages-derived conditioned medium (M1-CM and M2-CM). Data are presented as mean ± standard error of the mean (SEM) from a single experiment and are representative of at least two experiments. (D) Transwell invasion assay for SCC-25 cells at 48 hours post-incubation with monocyte- and macrophage-CM. Data are shown as mean ± SEM of cells counted in five representative microscopic fields per membrane using the ImageJ software. *P <0.05; **P<0.01
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microcal peaq itc integrated software  (Malvern Panalytical)
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Motility, proliferation, and invasiveness of SCC-25 cells after exposure to monocyte- and macrophages-conditioned medium. (A) Relative wound density curve of SCC-25 cells over 40 hours as measured by <t>IncuCyte</t> analysis. (B) Representative images of scratch assays showing wounds immediately after scratching (0h) and after 12 hours in the presence of monocyte- and macrophage-CM (right panels) versus control medium (left panels). Scale bars represent 300µm. (C) Proliferation curve for SCC-25 cells in the presence of monocyte and macrophages-derived conditioned medium (M1-CM and M2-CM). Data are presented as mean ± standard error of the mean (SEM) from a single experiment and are representative of at least two experiments. (D) Transwell invasion assay for SCC-25 cells at 48 hours post-incubation with monocyte- and macrophage-CM. Data are shown as mean ± SEM of cells counted in five representative microscopic fields per membrane using the ImageJ software. *P <0.05; **P<0.01
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Motility, proliferation, and invasiveness of SCC-25 cells after exposure to monocyte- and macrophages-conditioned medium. (A) Relative wound density curve of SCC-25 cells over 40 hours as measured by <t>IncuCyte</t> analysis. (B) Representative images of scratch assays showing wounds immediately after scratching (0h) and after 12 hours in the presence of monocyte- and macrophage-CM (right panels) versus control medium (left panels). Scale bars represent 300µm. (C) Proliferation curve for SCC-25 cells in the presence of monocyte and macrophages-derived conditioned medium (M1-CM and M2-CM). Data are presented as mean ± standard error of the mean (SEM) from a single experiment and are representative of at least two experiments. (D) Transwell invasion assay for SCC-25 cells at 48 hours post-incubation with monocyte- and macrophage-CM. Data are shown as mean ± SEM of cells counted in five representative microscopic fields per membrane using the ImageJ software. *P <0.05; **P<0.01
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Motility, proliferation, and invasiveness of SCC-25 cells after exposure to monocyte- and macrophages-conditioned medium. (A) Relative wound density curve of SCC-25 cells over 40 hours as measured by <t>IncuCyte</t> analysis. (B) Representative images of scratch assays showing wounds immediately after scratching (0h) and after 12 hours in the presence of monocyte- and macrophage-CM (right panels) versus control medium (left panels). Scale bars represent 300µm. (C) Proliferation curve for SCC-25 cells in the presence of monocyte and macrophages-derived conditioned medium (M1-CM and M2-CM). Data are presented as mean ± standard error of the mean (SEM) from a single experiment and are representative of at least two experiments. (D) Transwell invasion assay for SCC-25 cells at 48 hours post-incubation with monocyte- and macrophage-CM. Data are shown as mean ± SEM of cells counted in five representative microscopic fields per membrane using the ImageJ software. *P <0.05; **P<0.01
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Motility, proliferation, and invasiveness of SCC-25 cells after exposure to monocyte- and macrophages-conditioned medium. (A) Relative wound density curve of SCC-25 cells over 40 hours as measured by <t>IncuCyte</t> analysis. (B) Representative images of scratch assays showing wounds immediately after scratching (0h) and after 12 hours in the presence of monocyte- and macrophage-CM (right panels) versus control medium (left panels). Scale bars represent 300µm. (C) Proliferation curve for SCC-25 cells in the presence of monocyte and macrophages-derived conditioned medium (M1-CM and M2-CM). Data are presented as mean ± standard error of the mean (SEM) from a single experiment and are representative of at least two experiments. (D) Transwell invasion assay for SCC-25 cells at 48 hours post-incubation with monocyte- and macrophage-CM. Data are shown as mean ± SEM of cells counted in five representative microscopic fields per membrane using the ImageJ software. *P <0.05; **P<0.01
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image segmentation software vis visiopharm integrator system  (Visiopharm AS)
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Motility, proliferation, and invasiveness of SCC-25 cells after exposure to monocyte- and macrophages-conditioned medium. (A) Relative wound density curve of SCC-25 cells over 40 hours as measured by <t>IncuCyte</t> analysis. (B) Representative images of scratch assays showing wounds immediately after scratching (0h) and after 12 hours in the presence of monocyte- and macrophage-CM (right panels) versus control medium (left panels). Scale bars represent 300µm. (C) Proliferation curve for SCC-25 cells in the presence of monocyte and macrophages-derived conditioned medium (M1-CM and M2-CM). Data are presented as mean ± standard error of the mean (SEM) from a single experiment and are representative of at least two experiments. (D) Transwell invasion assay for SCC-25 cells at 48 hours post-incubation with monocyte- and macrophage-CM. Data are shown as mean ± SEM of cells counted in five representative microscopic fields per membrane using the ImageJ software. *P <0.05; **P<0.01
Image Segmentation Software Vis Visiopharm Integrator System, supplied by Visiopharm AS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Motility, proliferation, and invasiveness of SCC-25 cells after exposure to monocyte- and macrophages-conditioned medium. (A) Relative wound density curve of SCC-25 cells over 40 hours as measured by IncuCyte analysis. (B) Representative images of scratch assays showing wounds immediately after scratching (0h) and after 12 hours in the presence of monocyte- and macrophage-CM (right panels) versus control medium (left panels). Scale bars represent 300µm. (C) Proliferation curve for SCC-25 cells in the presence of monocyte and macrophages-derived conditioned medium (M1-CM and M2-CM). Data are presented as mean ± standard error of the mean (SEM) from a single experiment and are representative of at least two experiments. (D) Transwell invasion assay for SCC-25 cells at 48 hours post-incubation with monocyte- and macrophage-CM. Data are shown as mean ± SEM of cells counted in five representative microscopic fields per membrane using the ImageJ software. *P <0.05; **P<0.01

Journal: Journal of Applied Oral Science

Article Title: Monocytes and Macrophage-derived mediators influence the behavior of squamous cell carcinoma cell lines

doi: 10.1590/1678-7757-2025-0380

Figure Lengend Snippet: Motility, proliferation, and invasiveness of SCC-25 cells after exposure to monocyte- and macrophages-conditioned medium. (A) Relative wound density curve of SCC-25 cells over 40 hours as measured by IncuCyte analysis. (B) Representative images of scratch assays showing wounds immediately after scratching (0h) and after 12 hours in the presence of monocyte- and macrophage-CM (right panels) versus control medium (left panels). Scale bars represent 300µm. (C) Proliferation curve for SCC-25 cells in the presence of monocyte and macrophages-derived conditioned medium (M1-CM and M2-CM). Data are presented as mean ± standard error of the mean (SEM) from a single experiment and are representative of at least two experiments. (D) Transwell invasion assay for SCC-25 cells at 48 hours post-incubation with monocyte- and macrophage-CM. Data are shown as mean ± SEM of cells counted in five representative microscopic fields per membrane using the ImageJ software. *P <0.05; **P<0.01

Article Snippet: The proliferation was assessed by live-cell imaging (10× objective lens) using the IncuCyte ZOOM integrated software (Sartorius, Ann Arbor, MI, USA).

Techniques: Control, Derivative Assay, Transwell Invasion Assay, Incubation, Membrane, Software

Influence of monocyte, M1, and M2 macrophage-conditioned medium on Detroit 562 cell motility and proliferation. (A) Relative wound density curves for Detroit 562 cells over 40 hours as measured by IncuCyte analysis. (B) Representative images of scratch assays showing wounds immediately after scratching (0 hours) and after 12 hours in the presence of monocyte- and macrophage-derived conditioned medium (right panels) versus control medium (left panels). Scale bars represent 300 µm. (C) Proliferation curve for Detroit 562 cells in the presence of monocyte- and macrophage-derived conditioned medium. *P <0.05. Data are shown as mean ± SEM from a single experiment and are representative of at least two experiments.

Journal: Journal of Applied Oral Science

Article Title: Monocytes and Macrophage-derived mediators influence the behavior of squamous cell carcinoma cell lines

doi: 10.1590/1678-7757-2025-0380

Figure Lengend Snippet: Influence of monocyte, M1, and M2 macrophage-conditioned medium on Detroit 562 cell motility and proliferation. (A) Relative wound density curves for Detroit 562 cells over 40 hours as measured by IncuCyte analysis. (B) Representative images of scratch assays showing wounds immediately after scratching (0 hours) and after 12 hours in the presence of monocyte- and macrophage-derived conditioned medium (right panels) versus control medium (left panels). Scale bars represent 300 µm. (C) Proliferation curve for Detroit 562 cells in the presence of monocyte- and macrophage-derived conditioned medium. *P <0.05. Data are shown as mean ± SEM from a single experiment and are representative of at least two experiments.

Article Snippet: The proliferation was assessed by live-cell imaging (10× objective lens) using the IncuCyte ZOOM integrated software (Sartorius, Ann Arbor, MI, USA).

Techniques: Derivative Assay, Control